Journal: Environmental pollution (Barking, Essex : 1987)

Article Title: Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium.

doi: 10.1016/j.envpol.2022.119421

Figure Lengend Snippet: Fig. 3. The cytotoxicity of Cd and intracellular Cd accumulation with or without GOs pre-treatment. (a) The cellular viability of BEAS-2B cells detected by CCK8 assay after being treated to Cd (1, 5, 10, 20, 25, 30 and 50 μM) for 24 h (n = 5, * indicate P < 0.05, compared to the untreated group). (b) The heat map of the cell viability inspected by CCK8 assay for BEAS- 2B cells after being treated to Cd (1, 5, 10, 20, 25, 30 and 50 μM) for 24 h with or without pre-treatment of P-GO, A-GO, or G-GO at the dose of 10 μg/mL, respectively (n = 5). (c) Intracellular Cd mass quan tification by ICP-MS. BEAS-2B cells were pre-treated with P-GO, A-GO, G-GO at 10 μg/mL for 24 h, and then exposed to CdCl2 at 10 μM for 24 h (n = 5), *P < 0.05. (d) Western blot analysis of MT1M and MT2A protein expression levels in BEAS-2B cells exposed to either GOs (10 μg/mL) or Cd (10 μM) or a combina tion of both (GOs + Cd) for 24 h.

Article Snippet: Antibodies (Abs) used were as follows: anti-NRF2 Ab (1:2000 dilution, Proteintech, USA), anti-SOD1 Ab (1:5000 dilution, Proteintech, USA), anti-SOD2 Ab (1:3000 dilution, Proteintech, USA), anti-MT1M (1:500 dilution, Proteintech, USA), anti-MT2A Ab (1:2000 dilution, Affinity, USA), anti-Caspase-8 Ab (1:5000 dilution, Proteintech, USA), anti-Bax Ab (1:5000 dilution, Proteintech, USA), anti-Bcl-2 Ab (1:5000 dilution, Proteintech, USA), anti-RIP1 Ab (1:2500 dilution, Proteintech, USA), anti-RIP3 Ab (1:2500 dilution, Proteintech, USA), anti-ABCB1 Ab (1:5000 dilution, Proteintech, USA), anti-ABCC1 Ab (1:2500 dilution, Proteintech, USA), anti-ABCG2 Ab (1:1000 dilution, Proteintech, USA).

Techniques: CCK-8 Assay, Western Blot, Expressing

Journal: Poultry science

Article Title: The capability of L-carnitine-mediated antioxidant on cock during aging: evidence for the improved semen quality and enhanced testicular expressions of GnRH1, GnRHR, and melatonin receptors MT 1/2.

doi: 10.3382/ps/pez201

Figure Lengend Snippet: Figure 1. Immunohistochemistry of melatonin receptors in the testicular sections of cocks. The top raw (a, b, and c) shows melatonin receptors 1 (MT1), the middle raw (d, e and f) shows the melatonin receptors 2 (MT2), and the bottom row (g and h) shows the percentage of integrated optical density (IOD) for MT1-immunopositive and MT2-immunopositive, respectively. The spermatogenic cells show immunopositive reaction (arrows) in control (LC-0) and L-carnitine (LC) administration (LC-50, and LC-150) groups. The MT1-immunopositive signals shows high expressed in LC-150, moderate in LC-50, and low in LC-0, whereas MT2-immunopositive signals shows high expressed in LC-50, moderate in LC-150, and low in LC-0. (“∗∗” & “∗∗∗” means significant at P <0.01 and P <0.00, respectively. P < .05; Scale bar = 100 μm; n = 5).

Article Snippet: In brief, the tissue sections were incubated overnight at 4◦C with primary rabbit polyclonal MT1 antibody (Cat: 17,172– 1-AP) and rabbit polyclonal MT2 antibody (Cat: 20,809–1-AP) according to the manufacturer instructions (Proteintech, Wuhan Sanying Biological Technology Co. Ltd, China).

Techniques: Immunohistochemistry, Control